Effect of songga wood methanol extract (Strychnos ligustrina) on reducing IL-1β levels and free radical scavenger by FRAP method

Songa Songa wood (Strychnos ligustrida) is one of the endemic plants of Sumbawa island, West Nusa Tenggara province, Indonesia. This plant is widely used by the local community as a tonic, wound medicine, antimalarial, joint pain, diabetes, and anti-inflammatory. One of the causes of inflammation is free radicals. The amount of free radicals causes oxidative stress which can cause inflammation. The purpose of this study was to determine the activity of free radical Scavenger (FRAP) and the effect of giving methanol extract of songga wood (Strychnos ligustrina) on reducing IL-1β levels (anti-inflammatory) by Elisa method. Methanol extract of songga wood (S. ligustrina) was macerated with methanol (3 x 24hours). The test animals were divided into 6 groups: the normal group, negative control (Na-CMC 0.5%), positive control (Diclofenac sodium), and treatment with 3 dose variations of 50, 100, and 200mg/kgBW. The antioxidant activity test results of songga wood methanol extract IC₅₀ 78.05μg/mL and quercetin as a comparative control is 6.72μg/mL. The results of the anti-inflammatory activity test showed the value of IL-1β levels at doses of 50, 100, and 200mg/kgBW, namely 470.30, 205.99 and 135.75pg/mL, respectively. This study concludes that methanol extract of songga wood has strong antioxidant activity and has potential as an anti-inflammatory, with the most effective dose reducing IL-1β levels at 200mg/kgBW (p> 0.05).