Antioxidant and Sunscreen activity test of Hydrogel Methanol Fraction of Robusta Coffee Beans using In–vitro Method
Abstract
Coffee beans are rich in phenolic compounds such as phenolic acids, polyphenols and flavonoids which contain antioxidant activity. Robusta coffee (Coffea canephora) contains much higher levels of caffeine and chlorogenic acid than arabica coffee. The two compounds above have high antioxidant and UV filter activity so they can be formulated as sunscreen preparations. The aim of this study was to compare the antioxidant activity and sun protection factor of samples using green and roasted robusta coffee formulated into sunscreen hydrogel preparations from the methanol fraction of robusta coffee beans (Coffea canephora). Antioxidant activity testing using the DPPH (1,1-diphenyl-2- picrylhydrazyl) method at a wavelength of 515nm, showed the highest activity shown by the hydrogel preparation of the methanol fraction of green robusta coffee beans (Coffea canephora) with a free radical inhibition percentage value of 17.12ppm. Meanwhile, the percentage value of free radical inhibition from the hydrogel preparation from the methanol fraction of roasted robusta coffee beans (Coffea canephora) was 13.30ppm. The sun protection factor values for the methanol fraction hydrogel preparation of green and roasted robusta coffee beans (Coffea canephora) are 27.57 (ultra protection) and 10.66 (maximum protection).
Keywords
Antioxidant Activity, Sunscreen Activity, Coffee beans, Hydrogel