Unveiling the Antioxidant Power: Exploring Phytochemical Composition and In vitro Antioxidant Evaluation of Leaf Extract from Euclea racemosa

Euclea racemosa, belongs to family traditionally used for thr treatment of both infectious and noninfectious diseases and also reported that different bioactive compounds responsible for different pharmacological activity. The present study was to evaluate the phytochemical analysis and in vitro antioxidant activities of the crude leaf extracts of E. racemosa on the standard DPPH free radicals and total reducing power. The qualitative phytochemical findings revealed that the both ethyl acetate and ethanolic leaf extracts of E. racemosa shown the presence of secondary metabolites, including alkaloids, flavonoids, tannins and phenolic compounds, glycosides, and saponins. From the current results revealed that chloroform, ethyl acetate and ethanolic leaf extract of E. racemosa showed significant DPPH scavenging activity of 86.18%, 88.93% and 89.63% respectively at 100 µg/ml with IC₅₀ values of 21.71 µg/ml, 24.43 µg/ml, and 23.54 µg/ml respectively. In addition the total reducing power of the tested crude leaf extracts both chloroform (0.837) and ethyl acetate (0.962) leave extracts of showed promising reducing power than other two tested extracts. There for it was concluded that the chloroform and ethyl acetate leave extract of E. racemosa had significant DPPH radical scavenging activity and total ferric reducing power.