Design, Synthesis, Characterization and Antimicrobial, Anti-Inflammatory Activities of Novel Mannich Base Derivatives

The chief purpose of this study was to estimate the microbicidal and anti-inflammatory properties of novel mannich base analogues. These analogues were synthesised by condensation reaction between the active hydrogen donating compounds, guanidine and different aldehydes. These analogues were characterized by FT-IR, ¹³C-NMR, ¹H-NMR and Mass spectroscopy. Later the estimation of biological activities of these analogues was performed on anti-microbial and anti-inflammatory effects by using disc diffusion, insilico docking method, inhibition of protein denatured and membrane stabilizing activity methods. Each compound was shown admirable anti-microbial activities against E. coli, B. subtilis, A. flavus and A. niger. The anti-inflammatory activity result reveals an excellent effect on inhibition of protein denatured and RBC haemolysis was observed. The results obtained are firmly similar to insilico study results. These values were close to the reference drugs.