Formulation, Evaluation and Acute Oral Toxicity Study of Polyherbal Syrup TCZoAP

Polyherbalism is an ancient Ayurvedic concept for treating complex diseases. Nowadays, many CNS-associated complications are faced by people worldwide. Ayurvedic principles of polyherbalism were known for their effectiveness. In this study, a brain tonic was prepared as a polyherbal syrup formulation. Polyherbal syrup formulations were designed and evaluated. A total of five plants Zingiber officinale, Piper cubeba, Cinnamomum zeylanicum, Anacyclus pyrethrum, and Terminalia chebula were selected for formulation. These plants have already been used in many animal models for neuroprotective activity. After maceration, hydroalcoholic extracts were prepared and evaluated for phytochemical tests and IC50 values. The formulation was prepared and evaluated for various parameters like physical appearance, pH, density, viscosity, specific gravity, clarity test, and FTIR analysis of active phytoconstituents. After confirmation of quality control parameters, we performed acute oral toxicity (423), according to OECD guidelines. Based on this evaluation, we conclude that this polyherbal syrup formulation will be used in the future for preclinical evaluation of neuroprotection in various animal models to check pharmacological effects.