Combination of Zonisamide and α-Tocopherol in Pentylenetetrazol Induced Seizures using Zebra fish as Alternative Model

Epilepsy is a neurological disorder characterized by recurring spontaneous epileptic seizures. The present study was intended to establish combination of Zonisamide and α-tocopherol in Pentylenetetrazol (PTZ) induced seizures by using Zebra fish as alternative model. In PTZ induced model and T-maze test the fish were administered with Zonisamide 10mg/400 ml and α-tocopherol 20mg/400ml combination before being subjected to behavioral activity. The antieprleptic behavioural screening against PTZ induced seizures and cognitive dysfunction was determined using a T-maze test, accompanied by neurotransmitter estimation and antioxidant analysis. All the results were found to be significant. Zonisamide 10 mg/400 ml and α-tocopherol 20mg/400ml combination increased the locomotion activity. Zonisamide and α tocopherol treated group displayed memory impairments similar to PTZ group, taking a high/long time to reach the deepest chamber and spending more time in the incorrect arm. AEDs were discovered to modulate neurotransmitter levels, particularly GABA, in zebrafish brains. Biochemical parameters of various enzymes showed a favorable outcome with Zonisamide at concentrations of 10mg/400mL and α-tocopherol 20 mg/400 mL. Enzymes like SOD and Catalase were found to be significantly abundant in the brain whereas cortisol and MDA level was significantly reduced. Based on findings, we can conclude that the combination of zonisamide and α-tocopherol protects zebrafish from PTZ induced seizures behavioural and biochemical changes. Combination of Behavioral and biochemical information, makes this model a useful tool for future research and discovery of newer and safer AEDs.