Isolation and Characterization of Active Constituents Present in the Newly Discovered Crinum solapurense Plant

The aim of the current investigation was the isolation and characterization of two active constituents i.e. Gulonic acid and Shikimic acid from the leaf extract of the newly discovered medicinal plant of Crinum solapurense (Amaryllidaceae). The Crinum solapurense was collected from the local areas of Solapur, Maharashtra, India. The Gulonic acid and Shikimic acid were isolated using column-chromatography and TLC (Thin Layer Chromatography) and characterized by using UV spectroscopy, FTIR, ¹H-NMR, ¹³C-NMR, and GCMS. The isolated compounds showed positive results for various alkaloidal tests like Dragendroff’s, Mayer’s, Wagner’s and Hager’s tests. The first isolated active constituent i.e. Gulonic acid in UV spectroscopy showed ƛ max at 261 nm; in FTIR spectroscopy showed O-H stretching, aliphatic O-H, C=O carbonyl functional groups, in ¹H-NMR showed C-H group attached to –OH, C-H & -OH groups; ¹³C-NMR showed O-H stretching and carbon attached to –OH group and adjacent to carbonyl group; GCMS showed 196g/mol molecular weight. The second isolated active constituents i.e. Shikimic acid in UV spectroscopy showed ƛ max at 235nm; in FTIR spectroscopy showed O-H stretching, aliphatic O-H, C=C functional groups; in ¹H-NMR showed C-H group attached to methylene group, C-H group attached to C=C, C-H group attached to –OH, C-H group attached to methylene group; ¹³C-NMR showed C=O of –COOH group, C=C attached to –COOH group, carbon adjuvant to C=C; GCMS showed 181 g/mol molecular weight. These results showed that two isolated compounds i.e. Gulonic acid and Shikimic acid have never been reported from the leaves extract of the Crinum solapurense plant.