Research Journal of Pharmacy and Technology

The objective of study to presented detail pharmacognostic profile, phytochemical characterization and in vitro antioxidant activity of Cyphostemma auriculatum (Roxb.) with enormous therapeutic activities belongs to the family Vitaceae. The Leaves of plant were collected and the pharmacognostic nature and powder microscopy were performed.The Leaves are digitate, 5-foliolate. Petioles 4-12 cm long, terminal leaflet obovate to rhomboid or elliptic at base, serrate at margin, acute to short-acuminate at apex, 9-14 x 4.5-9.5 cm, petiolules 1-2 cm long. Microscopical identification of transverse section of the leaf has presence of covering trichomes and anomocytic stomata in the lower epidermis of the leaf. Upper epidermis cells are polygonal. Powder characteristic of leaves shows the presence of stomata, covering trichomes, annular vessels, acicular crystals of calcium oxalate in bundles and scattered form. The shade dried crude plant drug powder was tested for ash value, extractive values and loss on drying. The crude drug extracted with Petroleum ether and ethanol. The preliminary phytochemical test shows presence of flavonoids, alkaloids, tannins, carbohydrates, glycoside, triterpenoids, proteins, steroids. The petroleum ether and ethanolic extract of the leaves of Cyphostemma auriculatum (Roxb.) were tested for Total phenolic content and antioxidant activity using 1,1-diphenyl, 2-picryl hydrazyl (DPPH) free radical scavenging activity. In ethanolic extract showed the most significant activity of DPPH free radical scavenging activity than other extract. The results of the study can serve valuable source of information and provides suitable standard for identification of this plant material.