Research Journal of Pharmacy and Technology

This research investigates a sustainable synthesis of copper oxide (CuO) nanoparticles utilizing the aqueous leaf extract of Artabotrys hexapetalus as a reducing agent. A notable transition in color from green to brown during the synthesis signified the successful formation of CuO nanoparticles. FTIR proved the characteristic peaks between 408 and 700 cm⁻¹ indicated the presence of CuO nanoparticles. Additional peaks revealed functional groups (O-H, C=C, alkaloids, phenolics, halo compounds, and amines) essential for the nanoparticle reduction process. Zeta potential measured a negative surface charge of CuO with -1.41mV. According to SEM analysis, the biosynthesized CuO nanoparticles were found to have a primarily spherical morphology (25-30nm) with occasional voids (42 to 76nm), which suggests a porous architecture. Due to variations in the composition of their cell walls and electrostatic interactions, Gram-negative bacteria (Pseudomonas aeruginosa and Escherichia coli) were susceptible to the antibacterial effects of CuO nanoparticles. The results showed that combining 1 mg/1mL of CuO nanoparticles (225μg) with 1mg/1mL of IAA (225μg) significantly accelerated root elongation (7.5cm) and lessened the negative effects of the CuO nanoparticles. When CuO nanoparticles 150μg of 1mg/1 mL and IBA 150μg of 1mg/1mL were combined, shoot growth increased to 7.8cm. This study emphasizes the feasibility of producing eco-friendly nanoparticles from natural materials.