1Faculty of Pharmaceutical Science, Assam Down Town University, Sankar Madhab Path, Gandhi Nagar, Panikhaiti, Guwahati, Assam, India
2Berhampur University, Berhampur - 760007, Odisha, India
3RITEE College of Pharmacy, NH-6, Chhatauna, Mandir Hasaud, Raipur, Chhattisgarh, India
*Corresponding Author E-mail: uttampanigrahy@gmail.com
Online published on 19 December, 2025.
The quantification and analytical validation of Atomoxetine was developed with a stationary phase Phenomenex column C18 (4.6mm×250mm, 5μm) and LC1220 Agilent HPLC instrument equipped with variable wavelength detector at 270nm and Acetonitrile: Water containing 0.2% of triethylamine (85:15, v/v) was used as mobile phase passed at a flow rate 1mL/min. Elution takes place at retention time of 2.283min. Validation of method were performed as per ICH guidelines which shows linearity concentration range from 10-125μg/mL amid Correlation coefficient=0.999 with Y = 63164x+64858 regression equation obtained. LOD and LOQ of Atomoxetine were 0.07μg/mL and 0.21μg/mL correspondingly. Recovery of Atomoxetine was obtained 99.83-100.14%.
Atomoxetine, HPLC, Variable Wavelength Detector, ICH Guidelines
