Research Journal of Pharmacy and Technology

Antioxidants potential in plants are still widely explored, Ruellia tuberosa L. was a wild growing shrubs and traditionally used to lower blood sugar, treat wounds, and relieve pain. This plant has been underutilized in vitro and in silico research while having antioxidant activities. Testing the antioxidant activity of the extract and its fractions, doing in silico research, and qualitative and quantitative analysis of the phytochemical composition were the objectives of this work. Phytochemical analysis of the extract and its fractions was identified qualitatively by color testing and quantitatively by spectrophotometry. Antioxidant testing was carried out using the DPPH, FRAP, and ABTS assay methods. The active fraction was then identified by GC-MS to determine the content of secondary metabolites, which were then tested in silico along with software such as PyRx, Autodock Vina, and Discovery Studio 2021. Secondary metabolites were found in all samples’ qualitative phytochemical analysis results, and they were found in the extract and its fractions in different ways. Quantitatively, the largest TPC and TFC (161.18±9.70μg GAE/g and 224.74±10.16μg QE/g) and providing the strongest antioxidant activity were EAF with the DPPH method (55.51±2.52), Frap (53.55±2.89) and ABTS (42.77±2.13)μg/mL. In silico studies have shown that the compound. EAF is an active fraction containing the highest phenolic and flavonoid compounds and contributes directly to antioxidant activity. Meanwhile, by in silico approcah 6-Carbomethoxy-Trans-1-Oxaspiro[4.5]Decan-2,8-Dione presents opportunities for further development, but may require structural modifications to enhance its efficacy.