Diuretic Activity Assay of Ethyl Acetate Fraction of Star Fruit Wuluh Extract (Averhoa bilimbi L.) against Male White Rat (Rattus norvegicus)

Diuretics are compounds that can increase urine output. Empirically, people have used star fruit (Averrhoa bilimbi L.) as a diuretic. This study aims to determine the diuretic activity of ethyl acetate fraction of star fruit wuluh on the urine volume of rats. A total of 25 male white rats were divided into 5 treatment groups, with each group containing five animals. The first group was given 1% Na CMC suspension as a negative control, the second group was given a furosemide suspension dose of 3.6 mg/kg body weight as a positive control, the third group was given an ethyl acetate fraction dose of 0.44 g/kg body weight, the fourth group was given an ethyl acetate fraction dose of 0.88 g/kg body weight and the fifth group was given an ethyl acetate fraction dose of 1.76 g/kg body weight. All treatment is given orally. Diuretic activity test was conducted using the Lipschitz method, where 30 minutes before the test rats were induced with warm water as much as 1 mL/100 g body weight and urine volume measurements were taken every hour for 6 hours of observation the parameters observed were urine volume, percentage of urine excretion, diuretic action and diuretic activity. The results showed that the ethyl acetate fraction of carambola fruit at a dose of 1.76 g/kg body weight has a strong diuretic activity. The data obtained were also analyzed using two-way ANOVA test and followed by Tukey Post Hoc test. Based on the Tukey Post Hoc test results obtained the results obtained showed that the test preparation that has an effective dose is the ethyl acetate fraction group dose of 1.76 g/kg body weight and which shows that the time that gives the best diuretic effect is at 4 hours.