Antibreast Cancer and Antidiabetic Activity of Combination of Nymphae alba and Rhizophora mucronata Ethanolic Leaf Extract

The world's biggest cause of death for women is breast cancer. High glucose levels in diabetes mellitus can support the development of cancer. The use of traditional medicine is increasingly urgent due to its potential therapeutic benefits for the treatment of diabetes and cancer. White lotus (Nymphaea alba) and mangrove (Rhizophora mucronata) are aquatic plants that have many pharmacological activities, including antidiabetic, anticancer, and antioxidant. In this study, a combination of the two extracts was carried out to obtain better anticancer and antidiabetic activity. The combination of the extracts was mangrove: lotus = 25%: 75% (combination extract A), mangrove: lotus = 50%: 50% (combination extract B), and mangrove: lotus = 75%: 25% (combination extract C). The process involved macerating mangrove and lotus leaves with ethanol solvent, then combining the resulting extracts to create extracts A, B, and C. The biological activity of these three extracts, as well as the lotus and mangrove extracts, was subsequently examined in vitro test. Specifically, the resazurin method was used to assess the extracts' anticancer on MCF-7 cancer cells and antidiabetic effects toward the alpha glucosidase enzyme, respectively. The results of this study are that mangrove has the best antidiabetic and anticancer activity with IC50 values of 19.90 ± 0.62 and 50.11 µg/mL, respectively. The best combination of extracts is combination C with a ratio of mangrove and lotus extracts of 75%: 25% with IC50 antidiabetic and anticancer values of 1.68 ± 0.70 (highly active) and 76.26 µg/mL (active), respectively. The conclusion of this study is that the combination of white lotus leaf and mangrove extracts has great potential as an antidiabetic and anticancer agent.