To Develop and Validate RP-HPLC Analytical Technique for the Solid Dosage Forms of Dapagliflozin

The qualitative analysis of pharmaceuticals is a crucial part of the pharmaceutical industry's strategy for human safety. Developing a precise, consistent, and focused RP-HPLC stability test method for measuring Dapagliflozin in pharmaceutical dosage forms and API is the main objective of the ongoing efforts. The PDA detector was used for the inquiry, and the Agilent C18 column was used to obtain the separation required for this specific experiment. The mobile phase was a 30:10:60% v/v mixtures of sodium acetate buffer, THF, and methanol, and the effluent was measured at 223nm using a diode array detector. There was a 1 ml/min flow rate. The retention duration for Dapagliflozin was 6.5 minutes. After reviewing the literature, we found that , There was linearity in the range of for Dapagliflon, the correlation coefficient between 60 and 140 µg/ml (microgram/milliliter) was shown to be R2 = 0.99. The present method's accuracy was evaluated at 80%, 100%, and 120%. It was concluded that the Dapagliflozin test was accurate because the HPLC method produced 101.32% of the recoveries. Precision experiments revealed relative standard deviation values of less than two. The results from the previous data show that the RP-HPLC technique may be used successfully for the simultaneous assessment of Dapagliflozin in their formulation.