1Department of Pharmacology, Annamacharya College of Pharmacy, Boyanapally, Annamaiah Dist., Rajampet, Andhra Pradesh, India
2Department of Pharmacognosy, Principal, Professor, Annamacharya College of Pharmacy, Boyanapally, Annamaiah Dist., Rajampet, Andhra Pradesh, India
3Department of Pharmacology, University College of Pharmaceutical Sciences, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India
4Department of Pharmaceutics, Nirmala College of Pharmacy, Annamaiah Dist., Rajampet, Andhra Pradesh, India
*Corresponding Author E-mail: munrashaik12@gmail.com
**E-mail: dug.swarna@gmail.com
***E-mail: rahamathishaik040@gmail.com
The Gamma-oryzanol was assumed to have a protective effect against PTZ induced kindling in rats, Oxidative stress, inflammation in hippocampus, cell density in cortex.
To evaluate the Gammaoryzanol as Neuroprotective in rat model of Pentylenetetrazole induced kindling in Albino wistar rats.
Various equipment and apparatus were used, Centrifuge, UV-spectrophotometer and Homogeniser used to estimate antioxidant parameters. To determine how Gamma-Oryzanol affected the intensity and progression of seizures, kindling, cognitive impairment (Y-Maze,Elevated plus maze and open field) oxidative stress and brain damage in PTZ-induced kindling. Kindling was caused by alternate PTZ intraperitoneal injections of 10 injections. It was discovered that pretreatment with Gamma-Oryzanol 1 hour before the administration of PTZ and on a PTZ-free day resulted in a reduction in seizure score.
A mitigation of the progression of kindling, a decrease in transfer latency in elevated plus maze and an increase in the overall number of crossing, grossing in open field and increase in percentage alteration behavior in Y-Maze is observed. Gamma-Oryzanol lowered oxidative stress when compared to PTZ group, reduced gliosis in the hippocampus and improved cell density in the cortex.
These findings indicate the potential of Gamma-Oryzanol to act against PTZ-induced kindling, memory impairment, oxidative stress and histological aberrations.
Gamma-oryzanol, PTZ, Oxidative stress, Inflammation, Hippocampus and Cortex
