UV Spectrophotometric Method Development and Validation of Mupirocin in Bulk and Marketed Topical Formulation

Mupirocin is a topical antibiotic that provides effective treatment S. aureus and MRSA bacterial infections of the skin. MP exhibits effective antimicrobial activity against gram-positive and some gram-negative bacteria, but there are various articles proving their efficacy in the treatment of Impetigo, Folliculitis and Eczema.

The primary objective of this investigation was to develop and examine a simple, precise, resilient, sensitive, and reliable UV-spectroscopic technique for detecting Mupirocin in bulk and on the market topical preparations in accordance with ICH validation requirements.

A UV spectrophotometric method was developed to estimate Mupirocin, the solvent system used was Methanol and Distilled water (10:90v/v), with Mupirocin showing a λ_max at 221nm.

The technique demonstrated linearity within the range of 6 - 30µg/ml, R² value of 0.9981. The result indicated that LOD have been 0.32µg/ml and LOQ have 0.97µg/ml. The method demonstrated precision, accuracy, robustness, and specificity, with a %RSD of less than 2%. Stability studies showed that Mupirocin is unstable under acidic, alkaline, oxidative, and thermal conditions thus the need for stable formulations.

The demonstrated method's specificity, sensitivity, accuracy, preciseness, ruggedness, and robustness succeed in effectively assessing Mupirocin in bulk and marketed formulation, which facilitates its integration in a novel carrier system for increased topical therapeutic benefits.