1Plastic, Reconstructive and Aesthetic Surgery Division, Department of Surgery and Laboratory of Anatomy Faculty of Medicine, University of Jember, Jember, Indonesia.
2Faculty of Pharmacy, University of Jember, Indonesia.
3Biology Education Department, Faculty of Education, University of Jember, Indonesia.
*Corresponding Author E-mail: ulfa.fk@unej.ac.id
Hyperpigmentation is a common dermatological problem and manifests as darkening of the skin, which can negatively affect the quality of life. This condition is caused by the overproduction of melanin, which is synthesized in melanosomes through a process called melanogenesis. The enzyme involved in this process is tyrosinase, which catalyzes the chemical reactions leading to melanin production. Inhibiting the tyrosinase enzyme is a key strategy for treating hyperpigmentation. Common inhibitors include hydroquinone, retinoids, and glycolic acid, but long-term use of these compounds can cause side effects. Natural ingredients such as robusta coffee, which contains chlorogenic acid, have shown potential as tyrosinase inhibitors. Ethanol extracts of robusta coffee beans were found to contain chlorogenic acid (4.125%±0.068%) and exhibit tyrosinase inhibition activity, with an IC50 value of 553.531±9.485ppm. This activity is attributed to compounds such as chlorogenic acid, fatty acids, caffeine, and trigonelline present in coffee beans. The formulated gel and cream containing robusta coffee extract showed good physical properties, including homogeneity, viscosity, spreadability, and slightly acidic pH. Overall, this study highlights the potential of robusta coffee extract as a natural tyrosinase inhibitor to treat hyperpigmentation.
Chlorogenic acid, Coffea canephora, Melanogenesis, Thin-layer chromatography, Tyrosinase inhibitor
