1Dept. of Biotechnology, School of Applied Sciences, REVA University. Bengaluru. India.
2Dept. of Biotechnology, Nrupathunga University (Formerly Govt. Science College)Bengaluru. India.
*Corresponding Author E-mail: manjula.kr@reva.edu.in
Silver nanoparticles (AgNPs) synthesized via green methods have shown immense potential in biomedical applications due to their biocompatibility and multifunctional properties. This study investigates the antioxidant, antiproliferative, and apoptotic activities of biogenic silver nanoparticles synthesized from Stereospermum colais leaf extract. The nanoparticles were characterized using UV-Vis spectroscopy, SEM-EDX, FTIR, and XRD. Biological evaluations were conducted on SH-SY5Y neuroblastoma cells. The study focusing on antioxidant activity through DPPH assays, antiproliferative effects via MTT assays, and apoptotic gene expression analyzed using qPCR. The results revealed significant dose-dependent antioxidant and cytotoxic activities, with qPCR analysis indicating upregulation of pro-apoptotic genes and downregulation of anti-apoptotic markers. This study highlights the therapeutic potential of Stereospermum colais-derived AgNPs (ScAgNPs) as promising agents in oxidative stress and neuroblastoma management.
Silver nanoparticles, Stereospermum colais, Antioxidant activity, Apoptosis, SH-SY5Y cells, Cytotoxicity and qPCR
