Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2026
  • Volume: 19
  • Issue: 4

Optimization of polyphenol Extraction from Three Medicinal Plants Using Probe Sonication: ABox-Behnken Design and Response Surface Methodology Approach

  • Author:
  • Usha Kiran Reddy Thimmapuram1,2, Nischith Sirawase Shankar1, Maged Alkanad1,**, Annegowda Haradur Venkatappa1,*
  • Total Page Count: 10
  • Page Number: 1557 to 1566

1Department of Pharmacognosy, Sri. Adichunchanagiri College of Pharmacy, Adichunchanagiri University, B.g. Nagara, Mandya, Karnataka571448, India

2Faculty, Department of Pharmacogonosy, S V University College of Pharmaceutical sciences, S V University, Tirupati - 517502, Andra Pradesh, India

*Corresponding Author E-mail: drannegowda@accp.co.in

**magedalkanad@accp.co.in

Abstract

This study optimized probe sonication-assisted extraction of polyphenols from Terminalia bellerica, Kigelia pinnata, and Aerva lanata using Box-Behnken Design (BBD) and Response Surface Methodology (RSM). The effects of solvent composition (0-100% ethanol), sonication time (5-60min), and amplitude (10-98%) on total phenolic content (TPC) were evaluated. RSM analysis revealed optimal conditions: T. bellerica achieved maximum TPC (602.42±9.23µg GAE/mL) at 62.08% ethanol, 34.39min, and 62.37% amplitude; K. pinnata yielded 60.73±2.56µg GAE/mL at 44.26% ethanol, 27.03min, and 55.41% amplitude; and A. lanata showed highest TPC (34.24±1.28µg GAE/mL) at 46.54% ethanol, 20min, and 44.79% amplitude. The validated model confirmed probe sonication as an efficient, scalable method for polyphenol extraction, highlighting its potential for pharmaceutical and industrial applications.

Keywords

Terminalia bellerica, Kigelia pinnata, Aerva lanata, Polyphenols, Probe sonication, Response Surface Methodology, Box-Behnken Design