Assessment of Total Phenolic, Total Flavonoid and In Vitro Anti-Oxidant Potential of Water Extract of Ceropegia bulbosa var. Lushii (graham) hook. f. Tuber

The current study aims to assess the antioxidant properties of Ceropegia bulbosa var. lushii (Asclepiadaceae) utilizing in vitro models, specifically 2, 2-diphenyl-1-picrilhydrazyl (DPPH) radical scavenging activity, super oxide anion radical scavenging assay, and hydroxyl radical scavenging assay. Moreover, the total phenol and total flavonoid content were assessed using Folin-Ciocalteau reagent and colorimetric techniques with aluminum chloride respectively. Protein, carbohydrates, alkaloids, phenolic and flavonoid compounds were found in the preliminary phytochemical screening. The outcomes demonstrated that the extract from C. bulbosa tuber demonstrated DPPH radical scavenging action, with an IC₅₀ value of 167.32± 3 μg/mL, in contrast to the standard ascorbic acid's 122.87±1.448 μg/mL. The extract's IC₅₀ value for the super oxide anion radical scavenging activity was 1.75 ± 0.14 mg/ml, while the IC₅₀ value for the hydroxyl free radical scavenging assay was 125± 0.085μg/ml) compared to the standard Gallic acid (IC₅₀ = 2.999± 0.082μg/ml). The total phenolic and total flavonoid contents were reported as 53.33± 0.58 μg/mg extract and 141.66± 3.8μg/mg extract, respectively. With respect to the standard, the aqueous extract revealed a notable level of antioxidant activity. It has been revealed that the Ceropegia bulbosa tuber displays antioxidant activity. The additional research could be done to figure out the active principle producing this effect.