1Research Scholar, Goa College of Pharmacy, Panaji and Faculty at PES's Rajaram and Tarabai. Bandekar College of Pharmacy, Farmagudi Ponda, Goa University, Panaji, Goa, India.
2Dept. of Pharnaceutical Chemistry, Goa College of Pharmacy, Goa University, Panaji, Goa, India.
3Dept. of Pharnaceutical Chemistry, Goa College of Pharmacy, Goa University, Panaji, Goa, India.
*Corresponding Author E-mail: lotlikar.reema@gmail.com
A variety of compounds were synthesized using cephalosporin antibiotics namely cefotaxime sodium (CFX) and cefpodoxime proxetil (CPD) along with the metal ions from transition and alkaline earth metals. The wet reflux method was utilized, with potassium hydroxide serving as the base and methanol as the solvent. The characterization of the complexes was conducted using various physical and spectral techniques, which included evaluating color, decomposition temperatures, and infrared (IR) spectroscopy. Furthermore, elemental analysis was conducted to determine the percentages of carbon, hydrogen, and nitrogen. Atomic absorption spectroscopy was utilized to ascertain the metal composition, while X-ray diffraction offered insights into the structures of the complexes. The metal:ligand ratio was determined using Jobs method of continuous variation by UV-Visible spectrophotometry. Antibacterial activity is assessed using the Minimum Inhibitory Concentration (MIC99) against Escherichia coli, Staphylococcus aureus and Bacillus subtilis employing Mueller Hinton broth and the serial dilution method. The selected concentrations were then plated and incubated to count the number of viable colonies. The concentration that exhibited 99% inhibition of growth compared to the positive solvent control is considered the Minimum Inhibitory Concentration (MIC). The nickel complex of the antibiotics demonstrates significant and promising activity against the organisms.
Cefotaxime, Cefpodoxime, Antibacterial, MIC, Characterization
