1Gurunanak College of Pharmacy, Mauza-Nari, Near Dixit Nagar, Kamptee Road, Nagpur, 440026, Maharashtra State, India
2Department of Pharmacognosy, Krishna Institute of Pharmacy, Krishna Vishwa Vidyapeeth, Karad, 415539, Maharashtra, India
*Corresponding Author E-mail: suhaspadmane@yahoo.co.in
A derivative UV-spectrophotometric method has been developed and validated for estimation of ozenoxacin in bulk drug and cream formulations. The standard solution of ozenoxacin in 0.1N HCl was prepared and wavelength 245nm was selected for method development. The third order derivative spectrum was selected for determination of ozenoxacin in presence of preservative benzoic acid where the latter shows zero absorption at 245 nm. The linearity of the method was found in range 5-25µg/ml in 0.1N HCl with regression value of 0.9996. The recovery of ozenoxacin was found to be around 100%, indicating accuracy of the method. The method was found to be precise with maximum RSD of 1.176%. The LOD and LOQ were found to be 0.568µg/ml and 1.721µg/ml respectively. The developed method was specific for the estimation of ozenoxacin in the presence of preservative benzoic acid in cream formulation, and can be effectively used for routine quality control in industry.
Ozenoxacin, Benzoic acid, Third order derivative, UV-spectrophotometry, Validation
