1Department of Pharmaceutical Chemistry, Sree Chaitanya Institute of Pharmaceutical Sciences, LMD.Colony, Karimnagar-505527. AP
2Department of Pharmaceutical Chemistry, Sultan-Ul-Uloom College of Pharmacy, Mount Pleasant, Road# 3, Banjara Hills, Hyderabad-500 034
3University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, AP
A simple, precise, rapid and accurate reverse phase HPLC method developed for the estimation of Aprepitant in Capsules dosage form. An X-Terra RP C18, 250x4.6 mm, 5 μm partical size, with mobile phase consisting of 0.01 M Ammonium Acetate containing 0.1% Formic acid and Methanol in the ratio of 35:65 v/v was used. The flow rate was 1.5 ml/min and the effluents were monitored at 210 nm. The retention time was 13.7 min. The detector response was linear in the concentration of 14–168 μg/ml. The respective linear regression equation being Y=267157.4x+169105. The limit of detection and limit of quantification was 0.28 and 0.84 μg/ml respectively. The percentage assay of Aprepitant was 99.85%. The method was validated by determining its accuracy, precision and system suitability. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Aprepitant in bulk drug and in its pharmaceutical dosage form.
Aprepitant, RP-HPLC, Estimation, Capsuless
