Mitochondrial Anti-Oxidant Enzymes Caused by Cigarette Smoke in Experimental Wistar Rat

Cigarette smoking not only cause physiological damage to respiratory tract but also contributes to cardiovascular disease progression and to pathophysiological development of larynx, oral cavity, esophagus, pancreases, kidney, stomach, liver, and leukemia and colon cancer due depletion anti-oxidant enzymes. Wistar rats were divided into sham-operated control Group (I) (n = 6), Smoking group, exposed for 60 minutes every day in an inhalation chamber (10 liters) for the two week (14 days) Group (II) (n = 6). After sacrifice animals lung were removed and mitochondria were isolated. Mitochondrial antioxidant marker enzymes viz. Reduced Glutathione, Superoxide Dismutase and Catalase were measured and pathological changes were measured by histopathology. Smoking group was showed the depletion of anti-oxidant enzymes and severe degeneration of lung cells as compared to sham operated control rats.