University Dept. of Pharmaceutical Sciences, M.L.S. University, Udaipur, Rajasthan
*Corresponding Author E-mail: abhi181281@yahoo.com
Online published on 8 April, 2013.
An accurate, simple, reproducible and sensitive method for the determination of paracetamol was developed and validated. Paracetamol was identified using a, Microsorb-MV 100-5 C18 column (250 x 4.6 mm; North America) by binary gradient elution with a flow rate of 0.5 ml/min. The mobile phase composition was methanol: water (30:70) (v/v) and spectrophotometric detection was carried out at 245 nm. The linear range of determination for paracetamol was 0.816–250 μg/ml. The method was shown to be linear, reproducible, specific and sensitive.
Paracetamol, Reverse phase-High performance liquid chromatography, Validation
