Estimation of Diltiazem Hydrochloride from Tablet Formulation Using Spectrophotometric Methods

Three simple and sensitive visible spectrophotometric methods have been developed for the quantitative estimation of diltiazem hydrochloride (DITZ) from tablet dosage form. The developed methods are based on formation chloroform extractable colored complex of drug with metanil yellow (MY, method A), tropaeolin 000 (TRP, method B) and alizarin red (AR, method C). The colored complex formed in method A, B, and C showed absorbance maxima at 405.5, 484.5 and 426.0 nm and linearity was observed in the concentration range of 3–24 μg/ml, 3–24 μg/ml and 10–80 μg/ml of DITZ respectively. The results obtained were statistically validated and were found to be reproducible.