Development and Quantification 0f HPTLC Method for the Estimation of Kutkin in Picrorrhiza kurroa

A simple and reproducible high performance thin layer chromatography method was developed and validated for the estimation of kutkin in Picrorrhiza kurroa. The stationary phase used was precoated silica gel 60F254. The solvent system of chloroform: methanol (8.5:1.8, v/v) was used as mobile phase. the detection of spot was carried out at 258 nm. the method was validated in terms of linearity, accuracy, precision and specificity. The linearity was observed in the range of 480–1440 ng. The Kutkin content of 246.90 μg per 100mg was observed in the sample. The average percentage recovery value of 98.55% was obtained. The proposed method being precise and sensitive can be used for detection, monitoring and quantification of Kutkin in Picrorrhiza kurroa.