Stability Indicating RP-HPLC Method for Determination of Potassium Clavulanate in the presence of its Degradation Products

A stability indicating isocratic RP-LC method has been developed for the quantitative determination of Potassium Clavulanate in presence of its hydrolytic degraded products. Chromatographic separation was achieved on a C18 Nucleosil column with mobile phase consisting of 0.7% sodium dihydrogen phosphate and methanol in the ratio of 90: 10 v/v; retention time of the drug was 4.2±0.05 min, quantification was carried out at 230 nm with flow rate of 1 mL min⁻¹. In the developed LC method the resolution between potassium Clavulanate and products of degradation was found to be greater than 2 with r² value greater than 0.997.