Absorbance Correction and Vierodt's Spectrophotometric Methods for the Simultaneous Determination of Ofloxacin and Nitazoxanide in Combined Tablet Dosage Form

Two simple, accurate, precise, rapid and economical methods were developed for the simultaneous estimation of Nitazoxanide and Ofloxacin in tablet dosage form. First method is Vierodt's method (Method A), where the wavelenghths selected for quantitation were 295nm (λmax of Ofloxacin) and 347 nm (λmax of Nitazoxanide). Second method is absorbance correction method (Method B), it is based on correcting the absorbance of the component of interest from the total absorbance of the sample and the wavelengths used for estimation of both drugs were 295 nm and 380 nm. Both the methods were validated statistically and recovery studies were carried out. Both drugs show linearity in the concentration range of 2 – 30 μg/ml. Linearity of Nitazoxanide and Ofloxacin were in the range of ± 20% of the test concentration. The proposed methods have been applied successfully to the analysis of the cited drugs in pharmaceutical formulations with good accuracy and precision. The methods herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.