Stability Indicating RP-HPLC Method for Analysis of Ciprofloxacin in Tablet Dosage Form

A simple, sensitive, accurate, reproducible and stability indicating RP-HPLC method was developed for the rapid analysis of Ciprofloxacin in tablet dosage form. The chromatographic separation was performed on a RP C18 (250 x 4.6 i.d) mm, 5μm, column. The mobile phase of the method consists of equal amount of acetonitrile and methanol along with 1% acetic acid in aqueous solution in the ratio of 8:8:84 v/v in the flow rate of 1.5 mL/min. The retention time for ciprofloxacin is found to be 7.7min when monitored at 280 nm. The detector response was linear in the concentration range of 200–700 μg/mL. The limit of detection and limit of quantification was 15.145 and 44.784 μg/mL respectively. The percentage assay of Ciprofloxacin was 99.92%. Correlation coefficient (r) of the regression equation is more than 0.999. The precision of the method was established by intra and inter day assay values which were less than 1%. None of the degraded products interfered with the Ciprofloxacin peak thus ensuring stability indicating nature of the method.