Simultaneous Spectrophotometric Analysis of Glimiperide, Metformin and Piogilitazone HCl in Combined Dosage Form

Two simple, Specific, Accurate, and Precise spectrophotmetric methods were developed for simultaneous analysis of Glimiperide, Metformin and Piogilitazone HCl, in combined dosage form. The first method was based on simultaneous equation method, and the second method was based on Q-analysis (absorbance ratio method). Glimiperide has absorbance at 228nm, Metformin has absorbance at 237nm and Piogilitazone HCl has absorbance at 267nm in Methanol The linearity was obtained in concentration range of 2–20μgm/ml for all the drugs. In the first method, the concentrations of drugs were determined by using simultaneous equations, in the second method, the concentrations of drugs were determined by using ratio of absorbance at isoabsorptive point 257nm and 237 the λ_max MET. The results of analysis have been validated statistically and by recovery studies. The developed methods was found to be accurate, precise, selective and rapid for simultaneous spectrophotometric analysis of Glimiperide, Metformin and Piogilitazone HCl, in combined dosage form.