Research Journal of Pharmacy and Technology

SCOPUS
  • Year: 2010
  • Volume: 3
  • Issue: 4

Thidiazuron Induced Direct Regeneration from Leaf Explants of Scoparia dulcis L. – A Pharmaceutical Plant

  • Author:
  • Karthikeyan Subbarayan1,, Rajagopal Kalyanaraman1, Prathima Badrinarayanan1, Rajasekaran Murugan2, Gowri Karthik1, Gomathee Ravichandran1
  • Total Page Count: 4
  • DOI:
  • Page Number: 1099 to 1102

1Department of Biotechnology, School of Life Sciences, VELS University, Chennai-600117, India

2Group of Soil Biology and Plant Nutrition, Faculty of Organic Agricultural Sciences, University of Kassel, Nordbhanhof str 1a, D-37213, Witzenhausen, Germany

Abstract

Leaf explants of Scoparia dulcis L. gave rise to multiple shoots when cultured on MS medium supplemented with different concentrations of TDZ and IAA. The highest rate of shoot multiplication was obtained in MS medium containing 4.0 μM TDZ and 1.0 μM IAA (26.6 ± 0.98). Differentiated shoot buds elongated to 5.8 cm in 21 days in 9 μM KIN amended medium. The regenerated shoots were rooted on half-strength MS basal medium with different concentrations of IBA and IAA. The maximum number of roots was achieved on the medium containing 2.8 μM IBA. In vitro regenerated plantlets were transferred to plastic pots containing coco peat as a potting mix and were thereafter successfully established under ex vitro conditions. The survival percentage of transplanted plantlets was 90.6%.

Keywords

Scoparia dulcis, medicinal plant, leaf explants, micropropagation, Thidiazuron