Preparation and In-Vitro Evaluation of Abacavir Sulphate Loaded Microspheres Cross-Linked by Different Concentrations of Glutaraldehyde

Abacavir Sulphate loaded microspheres were formulated by using an enteric polymer Ethylcellulose by Solvent Evaporation Technique (SET) with HPMC 5C, Eudragit RSPO, and Eudragit RLPO to develop a sustained release dosage form. The effects of different concentration of cross-linking agent (Glutaraldehyde) on the percentage of drug loading, biodegradability of microspheres and drug release kinetics, particle size, entrapment efficiency, angle of repose, bulk density, SEM were investigated in this study. Moreover, the kinetics of Abacavir Sulphate released from different microspheres were analyzed using four different theoretical models, that is, Zero order, First order, Peppa's, and Higuchi models. Microspheres prepared with different concentration of Glutaraldehyde indicated different release kinetics. Increasing the Glutaraldehyde concentration decreased the release rate of Abacavir Sulphate from microspheres because of formation of greater structural strength and more tightly texture with the drug. Besides, microspheres gave an adequate fit to either zero order or first order kinetic models, depending on the extent of crosslinking reaction between drug and the cross linking agent.