Synthesis and Study of Some 1, 2, 4-Triazole derivatives

The 3-Hydroxy pyridine on reaction with ethylchloro acetate in presence of K₂CO₃ followed by the reaction with hydrazine hydrate resulted in the formation of 3-pyridoxy acetyl hydrazide (III), which on further the reaction with CS₂ and KOH gave potassium salt of thiosemicarbazide which on reaction with excess of hydrazine hydrate in ethanolic medium form 3-(3-pyridoxymethyl)-4-amino-5-mercapto-1,2,4-triazole (II). Compound (II) when condensed with various substituted benzaldehydes gives 3–(3–Pyridoxymethylene)–4–(N–substituted benzylidinylamino)–5–mercapto–1,2,4–triazole derivatives. These structures are determined by the elemental analysis and spectral data (IR, 1H-NMR). These new derivatives are evaluated for in vitro antimicrobial activity against Staphylococcus aureus (ATCC 3750), Salmonella typhi (NCTC 786), Candida albicans (ATCC 10231) and Aspergillus niger (ATCC 16404).