Validated RP-HPLC Method for the Quantitation of Nebivolol in Bulk and Pharmaceutical Dosage Forms

A simple, specific, accurate, precise and sensitive Reverse Phase High Performance Liquid Chromatographic method has been developed for the quantitation of Nebivolol in both pure and pharmaceutical dosage forms. A Phenomenex Gemini C-18, 5 μm column having 250 x 4.6 mm internal diameter in isocratic mode with mobile phase containing Acetonitrile: 50mM Ammonium acetate buffer (60: 40 v/v) and adjust the pH to 3.5 by using glacial acetic acid. The flow rate was 1.0 ml/min and the effluents were monitored at 282 nm. The retention time was 3.783 min. The linearity was in the range of 20–100 μg/ml. This method was validated for linearity, precision, limit of detection, limit of quantitation, accuracy, ruggedness and robustness. Statistical analysis proves that the method is reproducible and selective for the estimation of the said drug.