Spectrophotometric Estimation of Acebrophylline in Bulk and Capsule Formulation

Two simple, sensitive and accurate spectrophotometric methods have been developed for the estimation of Acebrophylline in bulk and in pharmaceutical formulations (Method A and Method B). Method A is based on the formation of red colored chromogen with ferric chloride in the presence of 1,10-phenanthroline and it obeys beer's law in the concentration ranging from 10–60μg/ml and exhibiting maximum absorption at 510nm. Method B is based on the formation of bluish green colored chromogen with ferric chloride in the presence of potassium ferricyanide and it obeys beer's law in the concentration ranging from 5–40μg/ml and exhibiting maximum absorption at 765nm. The methods were extended to capsule formulation and there was no interference from excipients and diluents. These methods have been statistically validated and are found to be precise and accurate.