Hydrotropic Solubilisation Technique of Metaxalone by Area Under Curve and Second Order Derivative Spectroscopy

Solubility of various poorly water-soluble drugs can be increased by the use of hydrotropic solubilisation technique. In the following investigation, solution of 0.5 M sodium acetate was employed as solublizing agent to solubilise poorly water soluble drug, Metaxalone showed absorbance maxima at 278nm. Beer's law is obeyed in the concentration range of 40–240μg/ml in two methods. Method-A (AUC) Area under curve which involves the calculation of integrated value of absorbance with respect to wavelength between 246-288nm. Method–B Derivative spectrocopy involves the derivatisation of the primary absorption spectra for the second order. The amplitude (D_L) of the long wave peak satellite of the second order curve was measured in mm. The results of analysis and the recovery studies which were validated statistically were found to be satisfactory. Presence of sodium acetate did not interfere in the analysis of Metaxalone and therefore both methods are accurate providing additional advantages of being cost effective and environmental friendly.