Simultaneous Determination of Nebivolol and Amlodipine in Combined Dosage Form by Derivative Spectrophotometry

Simple and accurate method to determine Nebivolol and Amlodipine, in tablet dosage form, was developed and validated using Derivative Spectrophotometry. Derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first-order derivative spectra were obtained at N = 1 (scaling factor), δλ = 2.0 nm, and determination was performed at 226.5 nm (ZCP of Amlodipine) for Nebivolol and 245 nm (ZCP of Nebivolol) for Amlodipine over the concentration range of 10–70 μg/mL for both drugs with mean recovery of 100.2 ± 1.25% and 100.1 ± 1.38% for Nebivolol and Amlodipine, respectively. Method was validated, and the results were compared statistically. They were found to be simple, sensitive, accurate, precise, reproducible and economical. The method was successfully applied for the determination of Nebivolol and Amlodipine in tablet dosage form without any interference from common excipients.