Development and Validation of Reverse Phase Liquid Chromatographic Methods for the Determination of Acebrophylline in Capsule Form.

A RP-HPLC method used for determination of acebrophylline in capsule was developed. Determination of acebrophylline in capsule was conducted by using RP-HPLC method with UV-Spectrophotometric detector at 240nm. Standards for calibration graph ranging from 200 to 600 μg/ml from stock solution. The proposed method was accurate with 98.7% recovery value and precisied with 0.902 relative standard deviation. These results were compared with that of standards operated with same RP-HPLC method. A reverse phase HPLC method with C18 column with ammonium orto-phosphate (0.01M)-acetonitrile-methanol(65:25:10)(pH-7)mobile phase was used and UV-detector was set to 240nm. Results obtained of test solution was compared to that of standard solution by using RP-HPLC.