Spectrophotometric Estimation of Granisetron in Bulk and Tablet Formulation

Three simple, precise and economical spectrophotometric methods have been developed for the estimation of granisetron in bulk and pharmaceutical preparations. Method A is based on the reaction of granisetron with Ferric chloride and 2, 2’ Bipyridyl to form blood red chromogen and followed by measuring the absorbance at 522nm. Method B is based on the reaction of granisetron with Ferric chloride and Potassium Ferricyanide to form bluish green chromogen and followed by measuring the absorbance at 820nm. Method C involves the determination of granisetron by absorbance ratio method. In this method the ratio of absorbances at any two wavelengths (301nm and 225nm) is constant for all concentrations were determined. The results of the analysis were validated statistically and found to be satisfactory.