A simple, sensitive and validated high performance thin layer chromatographic method has been developed for the estimation of Rupatadine Fumarate in pure drug and its formulation. Aluminium plates precoated with Silica gel G 60 F254 was used as stationary phase and Acetonitrile: Water: Formic acid in the ratio of 50:50:3 were used as mobile phase. Quantification was carried out by the use of Densitometric absorbance mode at 263 nm. The content of Rupatadine Fumarate in the formulation was calculated and found to be 99.1%. The proposed HPTLC method was quantitatively evaluated in terms of precision, repeatability, accuracy and calibration correlation proving its utility in routine analysis of its dosage form.
HPTLC, Densitometric Absorbance mode
