Simple and Sensitive LC-UV Method for Simultaneous Analysis of Lecarnidipine Hydrochloride and Atenolol in Pharmaceutical Formulations

A simple, sensitive and inexpensive high performance liquid chromatographic method has been developed for simultaneous determination of Atenolol and Lecarnidipine hydrochloride in pharmaceutical formulations. Chromatographic separation was achieved on a Phenomenex Gemini ODS column with a 75:25 mixture of methanol: water final P^H (4.0±0.1); as a mobile phase. Detection was at 274nm. The calibration curve was linear in the concentration range 60–180μg/ml for Atenolol 12–32μg/ml for Lecarnidipine Hydrochloride; the correlation coefficients were 0.9992 and 0.9991, repectively. Total elution time was less than 5 min.