Development of First Order Derivative UV –Spectrophotometric Method for the Estimation of Sitagliptin Phosphate

A simple, rapid, sensitive, economic and accurate first order derivative Spectrophotometric method has been developed for the estimation of Sitagliptin Phosphate in bulk and tablets. In distilled water λ_max of Sitagliptin Phosphate was found to be 267.4 nm. The same spectra were derivatized using derivative mode into first order derivative at δN =5, the amplitude of peak was measured at 256.4 nm. In the method linearity was observed in the concentration range of 5–35 μg/ml. the assay results by the proposed method were in good agreement with label claim, the method was validated statistically and recovery studies.