Evaluation and Comparison Study of Acetylcholine and Histamine on Isolated Chick, Rat, Guinea pig and Rabbit Ileum with Special Reference to PD₂ Value in Pharmacology Practical

In the present investigation sought to examine and compare the suitability of the easily available and economical Chick ileum with rat, guinea pig and rabbit ileum preparation as a teaching aid for pharmacology teachers for the purpose of Pharmacology Practicals. In this study the different preparations were screened against the common practical's like PD2 value of different drugs. All the isolated tissue preparation showed a significant response to most of the common spasmogens. It was observed chick ileum is particularly suitable for cholinergic receptors as comparison to histaminergic receptors. Rat ileum was suitable for cholinergic receptors as comparison to histaminergic receptors. Rabbit ileum produces good effect against both drugs, but histaminergic drugs produces better response. In this study it was found that students feel more comfort with chick ileum than the rat ileum, after chick ileum Students prefer rabbit ileum for Histaminergic practical. It was found chick and rabbit ileum suitable, and meant for UG Practicals for both Medical and pharmacy students in basic and preliminary practical. However, more studies required to compare all the effect in different isolated tissues and comparison study between chick and goat ileum though that are easily available from the slaughter house, and animals need not be killed just for practical purposes.