New Validated Method for the Determination of Memantine Hydrochloride Content in Tablet Dosage Forms by a High Performance Liquid Chromatography with Evaporative Light Scattering Detector

The aim of the present work was to develop and validate a liquid chromatographic method for the determination of memantine hydrochloride in tablet dosage form. Chromatographic separation was achieved on a intersil ODS 3v column (150×4.6 mm), 5 μm column with a HPLC grade methanol and 0.1% trifluroacetic acid buffer in the ratio of 50:50 (v/v/v) as mobile phase under ELS Detector conditions like a evaporation temperature 70°C, nebulization temperature 40°C, nitrogen flow rate 1.2SLM, and LED intensity 90%, injection volume is 20μL, flow rate is 1.0 mL/min and the expected retention of memantine hydrochloride peak is about 6.8 minutes. Response was a linear function of concentration in the range 2–0.01 μg/mL for memantine hydrochloride; correlation coefficiencent was 0.9998, respectively. LOD and LOQ for memantine hydrochloride were found 0.01 μg/mL and 0.03 μg/mL based on signal to noise ratio(S/N). Accuracy (recoveries 88–96%) and reproducibility were found to satisfactory. The present method can be applied successfully for the quantification of active pharmaceutical ingredient content in the combined formulations of memantine hydrochloride.