Simultaneous First Order Derivative Spectrophotometric Determination of Copper and Nickel Using Anthrone Phenylhydrazone (APH)

The authors prepared a new reagent namely anthrone phenylhydrazone (APH) is used for the analytical simultaneous determination of Cu(II) and Ni(II) by first order derivative spectrophotometric method. The reagent APH gives light yellow colour with Cu(II) and Ni(II) mixture solution in basic medium. The maximum peaks were observed between 350nm -410nm for Cu(II) and Ni(II) in basic buffer solution of pH 10.5. The molar absorptivity and sandell`s sensitivity of Cu(II) and Ni(II)-APH complexes are 5.065x10⁴L/mol/cm, 2.497x10⁴L/mol/cm and 0.0019μg/cm², 0.0042 μg/cm²respectively. The stability constant of Cu(II) and Ni(II)-APH complexes are 1.042x10⁵and 4.972x10⁴respectively. The effect of concentrations of Cu(II) and Ni(II) ions on amplitude are also studied. The samples of groundnut seeds, sesame seeds and grape leaf were analyzed by the proposed method. The results of the samples are good agreement with the results APARI and AAS methods.