Development of RP-HPLC Method for Simultaneous Estimation of Alprazolam and Fluoxetine Hydrochloride in Pharmaceutical Tablet Dosage Form

A new, simple, accurate and precise reversed phase high performance chromatographic method with UV detection was developed for the simultaneous quantitative estimation of Alprazolam (APZ) and Fluoxetine hydrochloride (FLX) in combined tablet dosage form. The method employed on reversed phase liquid chromatographic analysis using C18 column (4.6 × 250 mm, 5 μm). The Eluent was monitored by absorbance at wavelength 225 nm using a mixture of Acetonitrile and phosphate buffer (50:50 v/v) at pH 4.5 with a flow rate of 1.0 mL min⁻¹. Calibration curves were found to be linear in the concentration range of 0.5–2.00 μg mL⁻¹and 40–160 μg mL⁻¹for APZ and FLX respectively. The proposed method was validated by testing its Linearity, recovery, repeatability and it was successfully employed for the rapid and specific simultaneous quantitative estimation of APZ and FLX in combined tablet dosage form