Simultaneous Estimation of Donepezil and Memantine by Reverse Phase HPLC in Bulk and Pharmaceutical Dosage Form

This investigation describes a new precise, sensitive and accurate RP-HPLC method for the Simultaneous estimation of Donepezil and Memantine in Bulk and Tablets. The resolution of two drugs was achieved on Agilent C8 (150mm x 4.6mm i.d., 3.5mm particle size) column with UV detection at 230nm and the mobile phase consist of Buffer, water and Acetonitrile (50:5:45 v/v).Using chromatographic conditions described Donepezil and Memantine were well resolved with mean retention times of 3.707 and 6.976 min, respectively. Linear response (r>0.999) was observed over the range of 50–70μg/ml for Donepezil and 100–140μg/ml for Memantine. The lower limit of quantification and lower limit of detection was 9.95 and 3.0 for Donepezil and 10.01 and 3.14 for Memantine. The Validation parameters were performed according to the ICH guidelines and the proposed method can be useful in the routine analysis for the determination of Donepezil and Memantine in Pharmaceutical dosage forms.