Research Journal of Pharmacy and Technology
SCOPUS
  • Year: 2013
  • Volume: 6
  • Issue: 10

Development and Validation of RP-HPLC Method for Simultaneous Estimation of Cholecalcererol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in Powder Dosage Form

  • Author:
  • Shashikant D. Thorat, B. C. Ghodeswar, G. S. Darode, N. S. Shinde, S. R. Chaudhari
  • Total Page Count: 4
  • Page Number: 1107 to 1110

Department Pharmaceutical Chemistry, Amrutvahini Collage of Pharmacy, Sangamner, Dist-Ahmednagar, Maharashtra, India

*Corresponding Author E-mail: thorat_shashikant@ymail.com

Online published on 31 October, 2013.

Abstract

A simple, specific, accurate and precise Reverse Phase High Performance Liquid Chromatography method was developed for simultaneous estimation of Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) in powder dosage form on RP C-18 Column (EC 250/4 neucleosil 100-10) using Methanol: Water (97:3%v/v) as mobile phase. The flow rate was 1.0 ml/min and effluent was monitored at 274nm. The retention time for Cholecalceferol (Vitamin D3) and Tocopherol Acetate (Vitamin E) was found to be as 8.78 and 12.80 respectively. Proposed method was validated for Specificity, Precision, Accuracy, Linearity, and Robustness.

Keywords

Cholecalceferol, Tocopherol Acetate, Reverse Phase High Performance Liquid Chromatography