1Maratha Mandal's College of Pharmacy, Belgaum, Karnataka
2Research Scholar, Karpagam University, Coimbatore
3Department of Pharmacology, SET's College of Pharmacy, Dharwad-580 002, India
*Corresponding Author E-mail: vishnuak@rediffmail.com
Online published on 27 November, 2013.
Aristolochia indica root extract (alcoholic) was screened for its antitumor activity on proliferation of HT29 colon cancer cell line. The activity was determined by microculture tetrazolium viability (MTT) assay. The different concentrations (100, 50, 25, 12.5, 6.25 and 3.125 μg/ml) were taken and cells were exposed with the extract. The % cytotoxicity produced in MTT assay at100μg/ml was 69.21 with IC50 of 28.56. From these results it was observed that alcoholic extract of Aristolochia indica root has significant cytotoxic activity.
Aristolochia indica root, MTT assays, cytotoxicity, IC50
